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cpla 2 assay kit  (Cayman Chemical)


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    Cayman Chemical cpla 2 assay kit
    Cpla 2 Assay Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpla+2+assay+kit/cpla2+assay+kit/pmc11817744-184-14-18
    Average 90 stars, based on 1 article reviews
    cpla 2 assay kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Proximity Ligation Assay:

    Article Title: Four phospholipase A 2 genes encoded in the western flower thrips genome and their functional differentiation in mediating development and immunity
    Article Snippet: .. PLA 2 activities in whole bodies of 100 individuals at each stage (L2 larva, pupa, and adult) were measured using sPLA 2 and cPLA 2 assay kits (Cayman Chemical) containing arachidonyl thio-phosphatidyl choline (PC) and diheptanoyl thio-PC substrates, respectively, based on the method described by Vatanparast et al. . ..

    Article Title: Hypoxia-Induced Inflammation in In Vitro Model of Human Blood–Brain Barrier: Modulatory Effects of the Olfactory Ensheathing Cell-Conditioned Medium
    Article Snippet: .. For PLA 2 activity (cPLA 2 assay kit, Cayman, 765,021 Ann Arbor, MI, USA), equal amounts of cell HBMEC lysates were incubated in a 96-well plate with the substrate arachidonoyl thio-phosphatidylcholine (ATPC), as previously reported [ ]. ..

    Article Title: Maternal progesterone and adipose mPRε in pregnancy regulate the embryonic nutritional state
    Article Snippet: .. The supernatants were collected, and the PLA 2 activity was measured using the cPLA 2 Assay Kit (Cayman Chemical) following the manufacturer’s protocol. .. The cAMP concentration was determined by enzyme immunoassay (EIA) using a cAMP EIA kit (Cayman Chemical), following the manufacturer’s protocol.

    Activity Assay:

    Article Title: Hypoxia-Induced Inflammation in In Vitro Model of Human Blood–Brain Barrier: Modulatory Effects of the Olfactory Ensheathing Cell-Conditioned Medium
    Article Snippet: .. For PLA 2 activity (cPLA 2 assay kit, Cayman, 765,021 Ann Arbor, MI, USA), equal amounts of cell HBMEC lysates were incubated in a 96-well plate with the substrate arachidonoyl thio-phosphatidylcholine (ATPC), as previously reported [ ]. ..

    Article Title: An LPA species (18:1 LPA) plays key roles in the self-amplification of spinal LPA production in the peripheral neuropathic pain model
    Article Snippet: .. The protein concentration of the supernatant was determined by the Lowry method, and the assays were performed using a cPLA 2 assay kit (Cayman Chemicals, Ann Arbor, MI, USA) to evaluate the cPLA 2 activity or a modified cPLA 2 assay kit (Cayman Chemicals, Ann Arbor, MI, USA) to evaluate the iPLA 2 activity, as described previously [ ]. ..

    Article Title: Maternal progesterone and adipose mPRε in pregnancy regulate the embryonic nutritional state
    Article Snippet: .. The supernatants were collected, and the PLA 2 activity was measured using the cPLA 2 Assay Kit (Cayman Chemical) following the manufacturer’s protocol. .. The cAMP concentration was determined by enzyme immunoassay (EIA) using a cAMP EIA kit (Cayman Chemical), following the manufacturer’s protocol.

    Article Title: Thrombin Induced Apoptosis through Calcium-Mediated Activation of Cytosolic Phospholipase A 2 in Intestinal Myofibroblasts
    Article Snippet: .. STOP solution was then added to each well and absorbance read at 450 nm. cPLA 2 activity assay: cPLA 2 activity was analyzed with the cPLA 2 Assay Kit (Cayman Chemicals, Ann Arbor, MI, USA) according to the manufacturer’s instructions. ..

    Article Title: Impact of fluoroquinolones and aminoglycosides on P. aeruginosa virulence factor production and cytotoxicity
    Article Snippet: Statistical significance of differences was assessed using Student's t -tests for normally distributed data and performed using Prism 7 (GraphPad Software). .. ExoU sn-2 directed phospholipase activity was detected using an adapted Caymen chemical cPLA 2 assay kit in a 96-well plate format, as previously described [ ]. .. Assay conditions contained 1 mM Arachidonoyl thio-PC (ATPC) (Cayman Chemical, Michigan, U.S.A.), 1 μM PIP 2 (Avanti Polar Lipids, Alabama, U.S.A.), 25 μM mono ubiquitin (Merck), 2% DMSO (v/v) and 1.25 mM 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB) (Merck) in a final volume of 50 μl.

    Article Title: Glutamine deficiency shifts the asthmatic state toward neutrophilic airway inflammation
    Article Snippet: .. cPLA 2 activity in the lung homogenates was measured using a cPLA 2 assay kit, according to the manufacturer's instructions (765021, Cayman Chemical Company). ..

    Incubation:

    Article Title: Hypoxia-Induced Inflammation in In Vitro Model of Human Blood–Brain Barrier: Modulatory Effects of the Olfactory Ensheathing Cell-Conditioned Medium
    Article Snippet: .. For PLA 2 activity (cPLA 2 assay kit, Cayman, 765,021 Ann Arbor, MI, USA), equal amounts of cell HBMEC lysates were incubated in a 96-well plate with the substrate arachidonoyl thio-phosphatidylcholine (ATPC), as previously reported [ ]. ..

    Article Title: Polychlorinated Biphenyls Induce Cytotoxicity and Inflammation in an In Vitro Model of an Ocular Barrier
    Article Snippet: .. Lysates were incubated in a 96-well plate with the substrate arachidonoyl-thio-phosphatidylcholine (ATPC), using a cPLA 2 assay kit (Cayman Chemical, Ann Arbor, MI, USA), following the manufacturer’s instructions. ..

    Protein Concentration:

    Article Title: An LPA species (18:1 LPA) plays key roles in the self-amplification of spinal LPA production in the peripheral neuropathic pain model
    Article Snippet: .. The protein concentration of the supernatant was determined by the Lowry method, and the assays were performed using a cPLA 2 assay kit (Cayman Chemicals, Ann Arbor, MI, USA) to evaluate the cPLA 2 activity or a modified cPLA 2 assay kit (Cayman Chemicals, Ann Arbor, MI, USA) to evaluate the iPLA 2 activity, as described previously [ ]. ..

    Modification:

    Article Title: An LPA species (18:1 LPA) plays key roles in the self-amplification of spinal LPA production in the peripheral neuropathic pain model
    Article Snippet: .. The protein concentration of the supernatant was determined by the Lowry method, and the assays were performed using a cPLA 2 assay kit (Cayman Chemicals, Ann Arbor, MI, USA) to evaluate the cPLA 2 activity or a modified cPLA 2 assay kit (Cayman Chemicals, Ann Arbor, MI, USA) to evaluate the iPLA 2 activity, as described previously [ ]. ..



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    Variation of PLA 2 activities in response to specific inhibitors in F. occidentalis : bromoenol lactone (‘BEL’, iPLA 2 inhibitor), methylarachidonyl fluorophosphate (‘MAFP’, cPLA 2 inhibitor), and p -bromophenacyl bromide (‘BPB’, <t>sPLA</t> <t>2</t> inhibitor). ( a ) Variation in PLA 2 susceptibility to the inhibitors under arachidonate phospholipid (‘AA-PL’) substrate. ( b ) Variation in PLA 2 susceptibility to the inhibitors under non-arachidonate phospholipid (‘Non-AA-PL’). Enzyme extract was pre-incubated with each inhibitor for 15 min and the residual enzyme activity was estimated at 25 °C and pH 8.0. All treatments were replicated three times. Asterisk (*) indicates a significant difference at Type I error = 0.05 (LSD test) compared to control. ‘ns’ represents no significant difference.
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    Variation of PLA 2 activities in response to specific inhibitors in F. occidentalis : bromoenol lactone (‘BEL’, iPLA 2 inhibitor), methylarachidonyl fluorophosphate (‘MAFP’, cPLA 2 inhibitor), and p -bromophenacyl bromide (‘BPB’, <t>sPLA</t> <t>2</t> inhibitor). ( a ) Variation in PLA 2 susceptibility to the inhibitors under arachidonate phospholipid (‘AA-PL’) substrate. ( b ) Variation in PLA 2 susceptibility to the inhibitors under non-arachidonate phospholipid (‘Non-AA-PL’). Enzyme extract was pre-incubated with each inhibitor for 15 min and the residual enzyme activity was estimated at 25 °C and pH 8.0. All treatments were replicated three times. Asterisk (*) indicates a significant difference at Type I error = 0.05 (LSD test) compared to control. ‘ns’ represents no significant difference.
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    Image Search Results


    Variation of PLA 2 activities in response to specific inhibitors in F. occidentalis : bromoenol lactone (‘BEL’, iPLA 2 inhibitor), methylarachidonyl fluorophosphate (‘MAFP’, cPLA 2 inhibitor), and p -bromophenacyl bromide (‘BPB’, sPLA 2 inhibitor). ( a ) Variation in PLA 2 susceptibility to the inhibitors under arachidonate phospholipid (‘AA-PL’) substrate. ( b ) Variation in PLA 2 susceptibility to the inhibitors under non-arachidonate phospholipid (‘Non-AA-PL’). Enzyme extract was pre-incubated with each inhibitor for 15 min and the residual enzyme activity was estimated at 25 °C and pH 8.0. All treatments were replicated three times. Asterisk (*) indicates a significant difference at Type I error = 0.05 (LSD test) compared to control. ‘ns’ represents no significant difference.

    Journal: Scientific Reports

    Article Title: Four phospholipase A 2 genes encoded in the western flower thrips genome and their functional differentiation in mediating development and immunity

    doi: 10.1038/s41598-024-60522-8

    Figure Lengend Snippet: Variation of PLA 2 activities in response to specific inhibitors in F. occidentalis : bromoenol lactone (‘BEL’, iPLA 2 inhibitor), methylarachidonyl fluorophosphate (‘MAFP’, cPLA 2 inhibitor), and p -bromophenacyl bromide (‘BPB’, sPLA 2 inhibitor). ( a ) Variation in PLA 2 susceptibility to the inhibitors under arachidonate phospholipid (‘AA-PL’) substrate. ( b ) Variation in PLA 2 susceptibility to the inhibitors under non-arachidonate phospholipid (‘Non-AA-PL’). Enzyme extract was pre-incubated with each inhibitor for 15 min and the residual enzyme activity was estimated at 25 °C and pH 8.0. All treatments were replicated three times. Asterisk (*) indicates a significant difference at Type I error = 0.05 (LSD test) compared to control. ‘ns’ represents no significant difference.

    Article Snippet: PLA 2 activities in whole bodies of 100 individuals at each stage (L2 larva, pupa, and adult) were measured using sPLA 2 and cPLA 2 assay kits (Cayman Chemical) containing arachidonyl thio-phosphatidyl choline (PC) and diheptanoyl thio-PC substrates, respectively, based on the method described by Vatanparast et al. .

    Techniques: Incubation, Activity Assay

    Four PLA 2 genes ( Fo-PLA 2 A , Fo-PLA 2 B , Fo-PLA 2 C , Fo-PLA 2 D ) encoded in F. occidentalis genome and the variation in their expression profile. ( a ) Prediction of their functional domains using NCBI Conserved Domain Database (www. ncbi.nlm.nih.gov/cdd). ( b ) Classification of the four PLA 2 s with known PLA 2 s classified into secretory PLA 2 (sPLA 2 : I, III, and XII), calcium-dependent cellular PLA 2 (cPLA 2 : IV), calcium-independent cellular PLA 2 (PLA 2 : VI), lipoprotein-associated PLA 2 (Lp-PLA 2 : VIII), and lysosomal PLA 2 (LPLA 2 : XV). This phylogenetic analysis was performed using MEGA6.06. Bootstrapping values were obtained with 1000 iterations to support branching and clustering. Amino acid sequences of PLA 2 s were retrieved from GenBank with accession numbers shown in Table S1. ( c ) Expression patterns of the four PLA 2 s in different developmental stages of F. occidentalis . The heatmap was generated and the pattern analysis by a phylogenetic tree was performed using the ClustVis online tool ( https://biit.cs.us.ee/clustvis/ ).

    Journal: Scientific Reports

    Article Title: Four phospholipase A 2 genes encoded in the western flower thrips genome and their functional differentiation in mediating development and immunity

    doi: 10.1038/s41598-024-60522-8

    Figure Lengend Snippet: Four PLA 2 genes ( Fo-PLA 2 A , Fo-PLA 2 B , Fo-PLA 2 C , Fo-PLA 2 D ) encoded in F. occidentalis genome and the variation in their expression profile. ( a ) Prediction of their functional domains using NCBI Conserved Domain Database (www. ncbi.nlm.nih.gov/cdd). ( b ) Classification of the four PLA 2 s with known PLA 2 s classified into secretory PLA 2 (sPLA 2 : I, III, and XII), calcium-dependent cellular PLA 2 (cPLA 2 : IV), calcium-independent cellular PLA 2 (PLA 2 : VI), lipoprotein-associated PLA 2 (Lp-PLA 2 : VIII), and lysosomal PLA 2 (LPLA 2 : XV). This phylogenetic analysis was performed using MEGA6.06. Bootstrapping values were obtained with 1000 iterations to support branching and clustering. Amino acid sequences of PLA 2 s were retrieved from GenBank with accession numbers shown in Table S1. ( c ) Expression patterns of the four PLA 2 s in different developmental stages of F. occidentalis . The heatmap was generated and the pattern analysis by a phylogenetic tree was performed using the ClustVis online tool ( https://biit.cs.us.ee/clustvis/ ).

    Article Snippet: PLA 2 activities in whole bodies of 100 individuals at each stage (L2 larva, pupa, and adult) were measured using sPLA 2 and cPLA 2 assay kits (Cayman Chemical) containing arachidonyl thio-phosphatidyl choline (PC) and diheptanoyl thio-PC substrates, respectively, based on the method described by Vatanparast et al. .

    Techniques: Expressing, Functional Assay, Generated